Targeting Thrombopoietin/MPL Signaling to Mitigate JAK2V617F-Driven Cardiac Microvascular Disease.
Key claim: JAK2V617F-mutant blood cells drive cardiac microvascular disease under cardiometabolic stress via upregulated endocardial thrombopoietin/MPL signaling, which anti-MPL neutralizing antibody can reverse.
Abstract
BACKGROUND: Individuals with-mutant myeloproliferative neoplasms or clonal hematopoiesis of indeterminate potential have a markedly increased risk of cardiovascular disease, yet the mechanisms by which mutant blood cells drive vascular and cardiac dysfunction remain incompletely understood. Although the thrombopoietin receptor MPL is central to hematopoiesis and is expressed in vascular endothelial cells (ECs), its role in JAK2V617F-associated cardiovascular complications is unknown. METHODS: We generated chimeric mice with-mutant blood cells and wild-type endothelium by bone marrow transplantation and challenged them with a high-fat/high-cholesterol diet to model cardiometabolic stress. RESULTS: Mice with-mutant blood cells developed a distinct cardiovascular phenotype characterized by microvascular disease, increased left ventricular mass, and relatively preserved left ventricular ejection fraction. Histopathologic analysis revealed coronary arteriole stenosis, perivascular fibrosis, reduced microvascular density, and endocardial injury, without evidence of epicardial coronary stenosis or myocardial infarction. Single-cell RNA sequencing revealed activation of inflammatory, stress-response, and endothelial-to-mesenchymal transition gene signatures in ECs, most prominently within the endocardial ECs. Immunohistochemistry identified MPL expression predominantly in endocardial ECs. Thrombopoietin/MPL signaling was upregulated in endocardial ECs in mice with-mutant hematopoiesis, and treatment with an anti-MPL neutralizing antibody markedly improved cardiovascular pathology, restored endocardial integrity, and increased coronary microvascular density. CONCLUSIONS: -mutant hematopoiesis induces cardiac microvascular dysfunction under cardiometabolic stress. Endocardial ECs play a critical role in this pathological process, and endocardial MPL signaling constitutes a potential targetable pathway in JAK2V617F-associated cardiovascular disease.
Why this matters
This preclinical finding identifies cardiometabolic stress as the trigger that unmasks JAK2V617F-driven cardiac microvascular disease, which is one of the few concrete mechanistic links between a modifiable lifestyle-influenced state (metabolic health, diet, adiposity) and MPN-specific end-organ damage. It doesn’t establish that any dietary intervention changes outcomes in MPN patients, but it strengthens the biological rationale for prioritizing cardiometabolic risk management in JAK2V617F carriers. The therapeutic angle (anti-MPL antibody) is outside the lifestyle scope.